Shotgun glycomics of pig lung identifies natural endogenous receptors for influenza viruses.

ABSTRACT: Influenza viruses bind to host cell surface glycans containing terminal sialic acids, but as studies on influenza binding become more sophisticated, it is becoming evident that although sialic acid may be necessary, it is not sufficient for productive binding. To better define endogenous glycans that serve as viral receptors, we have explored glycan recognition in the pig lung, because influenza is broadly disseminated in swine, and swine have been postulated as an intermediary host for the emergence of pandemic strains. For these studies, we used the technology of "shotgun glycomics" to identify natural receptor glycans. The total released N- and O-glycans from pig lung glycoproteins and glycolipid-derived glycans were fluorescently tagged and separated by multidimensional HPLC, and indiv

SUBMITTER: Byrd-Leotis L 

PROVIDER: S-EPMC4050609 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature