A crucial role for the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification.

ABSTRACT: Mammalian development is regulated by the interplay of tissue-specific and ubiquitously expressed transcription factors, such as Sp1. Sp1 knockout mice die in utero with multiple phenotypic aberrations, but the underlying molecular mechanism of this differentiation failure has been elusive. Here, we have used conditional knockout mice as well as the differentiation of mouse ES cells as a model with which to address this issue. To this end, we examined differentiation potential, global gene expression patterns and Sp1 target regions in Sp1 wild-type and Sp1-deficient cells representing different stages of hematopoiesis. Sp1(-/-) cells progress through most embryonic stages of blood cell development but cannot complete terminal differentiation. This failure to fully differentiate is not seen

SUBMITTER: Gilmour J 

PROVIDER: S-EPMC4050696 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature