Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus.

ABSTRACT:

Background

The Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rfp have been used in hybrid breeding in Brassica napus, which has greatly improved the yield of rapeseed. However, the mechanism of the male sterility transition in pol CMS remains to be determined.

Results

To investigate the transcriptome during the male sterility transition in pol CMS, a near-isogenic line (NIL) of pol CMS was constructed. The phenotypic features and sterility stage were confirmed by anatomical analysis. Subsequently, we compared the genomic expression profiles of fertile and sterile young flower buds by RNA-Seq. A total of 105,481,136 sequences were successfully obtained. These reads were assembled into 112,770 unigenes, which composed the transcript

SUBMITTER: An H 

PROVIDER: S-EPMC4051170 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature