Mapping in an apple (Malus x domestica) F1 segregating population based on physical clustering of differentially expressed genes.

ABSTRACT:

Background

Apple tree breeding is slow and difficult due to long generation times, self-incompatibility, and complex genetics. The identification of molecular markers linked to traits of interest is a way to expedite the breeding process. In the present study, we aimed to identify genes whose steady-state transcript abundance was associated with inheritance of specific traits segregating in an apple (Malus × domestica) rootstock F1 breeding population, including resistance to powdery mildew (Podosphaera leucotricha) disease and woolly apple aphid (Eriosoma lanigerum).

Results

Transcription profiling was performed for 48 individual F1 apple trees from a cross of two highly heterozygous parents, using RNA isolated from healthy, actively-growing shoot tips and a custom apple DN

SUBMITTER: Jensen PJ 

PROVIDER: S-EPMC4051173 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature