Structure solution of DNA-binding proteins and complexes with ARCIMBOLDO libraries.

ABSTRACT: Protein-DNA interactions play a major role in all aspects of genetic activity within an organism, such as transcription, packaging, rearrangement, replication and repair. The molecular detail of protein-DNA interactions can be best visualized through crystallography, and structures emphasizing insight into the principles of binding and base-sequence recognition are essential to understanding the subtleties of the underlying mechanisms. An increasing number of high-quality DNA-binding protein structure determinations have been witnessed despite the fact that the crystallographic particularities of nucleic acids tend to pose specific challenges to methods primarily developed for proteins. Crystallographic structure solution of protein-DNA complexes therefore remains a challenging area that i

SUBMITTER: Propper K 

PROVIDER: S-EPMC4051508 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature