High expression of 23 kDa protein of augmenter of liver regeneration (ALR) in human hepatocellular carcinoma.

ABSTRACT:

Background

Augmenter of liver regeneration (ALR) is an important polypeptide that participates in the process of liver regeneration. Two forms of ALR proteins are expressed in hepatocytes. Previous data have shown that ALR is essential for cell survival and has potential antimetastatic properties in hepatocellular carcinoma (HCC).

Aims

The study aimed to evaluate the expression levels of two forms of ALR proteins in HCC and their possible significance in HCC development.

Methods

Balb/c mouse monoclonal antibody against ALR protein was prepared in order to detect the ALR protein in HCC by Western blotting and immunohistochemistry. ALR mRNA expression levels were measured by real-time polymerase chain reaction in HCC tissues and compared to paracancerous liver tissues i

SUBMITTER: Yu HY 

PROVIDER: S-EPMC4051792 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature