Role of the Na ⁺/K ⁺/2Cl⁻ cotransporter NKCC1 in cell cycle progression in human esophageal squamous cell carcinoma.

ABSTRACT:

Aim

To investigate the role of Na(+)/K(+)/2Cl(-) cotransporter 1 (NKCC1) in the regulation of genes involved in cell cycle progression and the clinicopathological significance of its expression in esophageal squamous cell carcinoma (ESCC).

Methods

An immunohistochemical analysis was performed on 68 primary tumor samples obtained from ESCC patients that underwent esophagectomy. NKCC1 expression in human ESCC cell lines was analyzed by Western blotting. Knockdown experiments were conducted using NKCC1 small interfering RNA, and the effects on cell cycle progression were analyzed. The gene expression profiles of cells were analyzed by microarray analysis.

Results

Immunohistochemical staining showed that NKCC1 was primarily found in the cytoplasm of carcinoma cells and th

SUBMITTER: Shiozaki A 

PROVIDER: S-EPMC4051924 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature