Ultimate use of two-photon fluorescence microscopy to map orientational behavior of fluorophores.

ABSTRACT: The orientational distribution of fluorophores is an important reporter of the structure and function of their molecular environment. Although this distribution affects the fluorescence signal under polarized-light excitation, its retrieval is limited to a small number of parameters. Because of this limitation, the need for a geometrical model (cone, Gaussian, etc.) to effect such retrieval is often invoked. In this work, using a symmetry decomposition of the distribution function of the fluorescent molecules, we show that polarized two-photon fluorescence based on tunable linear dichroism allows for the retrieval of this distribution with reasonable fidelity and without invoking either an a priori knowledge of the system to be investigated or a geometrical model. We establish the optimal

SUBMITTER: Ferrand P 

PROVIDER: S-EPMC4052276 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature