Independent synchronized control and visualization of interactions between living cells and organisms.

ABSTRACT: To investigate the early stages of cell-cell interactions occurring between living biological samples, imaging methods with appropriate spatiotemporal resolution are required. Among the techniques currently available, those based on optical trapping are promising. Methods to image trapped objects, however, in general suffer from a lack of three-dimensional resolution, due to technical constraints. Here, we have developed an original setup comprising two independent modules: holographic optical tweezers, which offer a versatile and precise way to move multiple objects simultaneously but independently, and a confocal microscope that provides fast three-dimensional image acquisition. The optical decoupling of these two modules through the same objective gives users the possibility to easily i

SUBMITTER: Rouger V 

PROVIDER: S-EPMC4052360 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature