Genome-wide polymorphisms and development of a microarray platform to detect genetic variations in Plasmodium yoelii.

ABSTRACT: The rodent malaria parasite Plasmodium yoelii is an important model for studying malaria immunity and pathogenesis. One approach for studying malaria disease phenotypes is genetic mapping, which requires typing a large number of genetic markers from multiple parasite strains and/or progeny from genetic crosses. Hundreds of microsatellite (MS) markers have been developed to genotype the P. yoelii genome; however, typing a large number of MS markers can be labor intensive, time consuming, and expensive. Thus, development of high-throughput genotyping tools such as DNA microarrays that enable rapid and accurate large-scale genotyping of the malaria parasite will be highly desirable. In this study, we sequenced the genomes of two P. yoelii strains (33X and N67) and obtained a large number of s

SUBMITTER: Nair SC 

PROVIDER: S-EPMC4052438 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature