ERbeta1 represses basal breast cancer epithelial to mesenchymal transition by destabilizing EGFR.

ABSTRACT:

Introduction

Epithelial to mesenchymal transition (EMT) is associated with the basal-like breast cancer phenotypes. 60% of basal-like cancers have been shown to express wild-type estrogen receptor beta (ERbeta1). However, it is still unclear whether the ERbeta expression is related to EMT, invasion and metastasis in breast cancer. In the present study, we examined whether ERbeta1 through regulating EMT can influence invasion and metastasis in basal-like cancers.

Methods

Basal-like breast cancer cells (MDA-MB-231 and Hs578T) in which ERbeta1 was either overexpressed or downregulated were analyzed for their ability to migrate and invade (wound-healing assay, matrigel-coated Transwell assay) as well as for the expression of EMT markers and components of the EGFR pathway (immuno

SUBMITTER: Thomas C 

PROVIDER: S-EPMC4053135 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature