Purification, characterization, and potential of saline waste water remediation of a polyextremophilic α-amylase from an obligate halophilic Aspergillus gracilis.

ABSTRACT: An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa. The specific activity of the enzyme was found to be 131.02 U/mg. The Lineweaver-Burk plot showed the V max and K m values of 8.36 U/mg and 6.33 mg/mL, respectively. The enzyme was found to have the best activity at 5 pH, 60°C, and 30% of NaCl concentration, showing its polyextremophilic nature. The use of various additives did not show much variation in the activity

SUBMITTER: Ali I 

PROVIDER: S-EPMC4053144 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature