Genome-wide co-localization of Polycomb orthologs and their effects on gene expression in human fibroblasts.

ABSTRACT:

Background

Polycomb group proteins form multicomponent complexes that are important for establishing lineage-specific patterns of gene expression. Mammalian cells encode multiple permutations of the prototypic Polycomb repressive complex 1 (PRC1) with little evidence for functional specialization. An aim of this study is to determine whether the multiple orthologs that are co-expressed in human fibroblasts act on different target genes and whether their genomic location changes during cellular senescence.

Results

Deep sequencing of chromatin immunoprecipitated with antibodies against CBX6, CBX7, CBX8, RING1 and RING2 reveals that the orthologs co-localize at multiple sites. PCR-based validation at representative loci suggests that a further six PRC1 proteins have similar bin

SUBMITTER: Pemberton H 

PROVIDER: S-EPMC4053772 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature