Extensive localization of long noncoding RNAs to the cytosol and mono- and polyribosomal complexes.

ABSTRACT:

Background

Long noncoding RNAs (lncRNAs) form an abundant class of transcripts, but the function of the majority of them remains elusive. While it has been shown that some lncRNAs are bound by ribosomes, it has also been convincingly demonstrated that these transcripts do not code for proteins. To obtain a comprehensive understanding of the extent to which lncRNAs bind ribosomes, we performed systematic RNA sequencing on ribosome-associated RNA pools obtained through ribosomal fractionation and compared the RNA content with nuclear and (non-ribosome bound) cytosolic RNA pools.

Results

The RNA composition of the subcellular fractions differs significantly from each other, but lncRNAs are found in all locations. A subset of specific lncRNAs is enriched in the nucleus but surpr

SUBMITTER: van Heesch S 

PROVIDER: S-EPMC4053777 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature