Global regulation of mRNA translation and stability in the early Drosophila embryo by the Smaug RNA-binding protein.

ABSTRACT:

Background

Smaug is an RNA-binding protein that induces the degradation and represses the translation of mRNAs in the early Drosophila embryo. Smaug has two identified direct target mRNAs that it differentially regulates: nanos and Hsp83. Smaug represses the translation of nanos mRNA but has only a modest effect on its stability, whereas it destabilizes Hsp83 mRNA but has no detectable effect on Hsp83 translation. Smaug is required to destabilize more than one thousand mRNAs in the early embryo, but whether these transcripts represent direct targets of Smaug is unclear and the extent of Smaug-mediated translational repression is unknown.

Results

To gain a panoramic view of Smaug function in the early embryo, we identified mRNAs that are bound to Smaug using RNA co-immunoprec

SUBMITTER: Chen L 

PROVIDER: S-EPMC4053848 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature