Role of norEF in denitrification, elucidated by physiological experiments with Rhodobacter sphaeroides.

ABSTRACT: Many denitrifying organisms contain the norEF gene cluster, which codes for two proteins that are thought to be involved in denitrification because they are expressed during the reduction of nitrite and nitric oxide. The products of both genes are predicted to be membrane associated, and the norE product is a member of the cytochrome c oxidase subunit III family. However, the specific role of norEF is unknown. The denitrification phenotypes of Rhodobacter sphaeroides strains with and without norEF genes were studied, and it was found that loss of norEF lowered the rate of denitrification from nitrate and resulted in accumulation of micromolar concentrations of nitric oxide during denitrification from nitrite. norEF appears to have no direct role in the reduction of nitric oxide; however, s

SUBMITTER: Bergaust LL 

PROVIDER: S-EPMC4054195 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature