Reconstruction of mreB expression in Staphylococcus aureus via a collection of new integrative plasmids.

ABSTRACT: Protein localization has been traditionally explored in unicellular organisms, whose ease of genetic manipulation facilitates molecular characterization. The two rod-shaped bacterial models Escherichia coli and Bacillus subtilis have been prominently used for this purpose and have displaced other bacteria whose challenges for genetic manipulation have complicated any study of cell biology. Among these bacteria is the spherical pathogenic bacterium Staphylococcus aureus. In this report, we present a new molecular toolbox that facilitates gene deletion in staphylococci in a 1-step recombination process and additional vectors that facilitate the insertion of diverse reporter fusions into newly identified neutral loci of the S. aureus chromosome. Insertion of the reporters does not add any ant

SUBMITTER: Yepes A 

PROVIDER: S-EPMC4054220 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature