Mutations in HIV-1 reverse transcriptase affect the errors made in a single cycle of viral replication.

ABSTRACT:

Unlabelled

The genetic variation in HIV-1 in patients is due to the high rate of viral replication, the high viral load, and the errors made during viral replication. Some of the mutations in reverse transcriptase (RT) that alter the deoxynucleoside triphosphate (dNTP)-binding pocket, including those that confer resistance to nucleoside/nucleotide analogs, affect dNTP selection during replication. The effects of mutations in RT on the spectrum (nature, position, and frequency) of errors made in vivo are poorly understood. We previously determined the mutation rate and the frequency of different types of mutations and identified hot spots for mutations in a lacZα (the α complementing region of lacZ) reporter gene carried by an HIV-1 vector that replicates using wild-type RT. We show

SUBMITTER: Abram ME 

PROVIDER: S-EPMC4054409 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature