Mechanism and significance of cell type-dependent neutralization of flaviviruses.

ABSTRACT:

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The production of neutralizing antibodies (NAbs) is a correlate of protection for many human vaccines, including currently licensed vaccines against flaviviruses. NAbs are typically measured using a plaque reduction neutralization test (PRNT). Despite its extensive use, parameters that impact the performance of the PRNT have not been investigated from a mechanistic perspective. The results of a recent phase IIb clinical trial of a tetravalent dengue virus (DENV) vaccine suggest that NAbs, as measured using a PRNT performed with Vero cells, do not correlate with protection. This surprising finding highlights the importance of understanding how well the PRNT captures the complexity of the NAb response to DENV. In this study, we demonstrated that the structural heterogeneit

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC4054442 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature