Evidence for the biogenesis of more than 1,000 novel human microRNAs.

ABSTRACT:

Background

MicroRNAs (miRNAs) are established regulators of development, cell identity and disease. Although nearly two thousand human miRNA genes are known and new ones are continuously discovered, no attempt has been made to gauge the total miRNA content of the human genome.

Results

Employing an innovative computational method on massively pooled small RNA sequencing data, we report 2,469 novel human miRNA candidates of which 1,098 are validated by in-house and published experiments. Almost 300 candidates are robustly expressed in a neuronal cell system and are regulated during differentiation or when biogenesis factors Dicer, Drosha, DGCR8 or Ago2 are silenced. To improve expression profiling, we devised a quantitative miRNA capture system. In a kidney cell system, 400 ca

SUBMITTER: Friedlander MR 

PROVIDER: S-EPMC4054668 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature