A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector.

ABSTRACT:

Background

Metabolic resistance to insecticides is the biggest threat to the continued effectiveness of malaria vector control. However, its underlying molecular basis, crucial for successful resistance management, remains poorly characterized.

Results

Here, we demonstrate that the single amino acid change L119F in an upregulated glutathione S-transferase gene, GSTe2, confers high levels of metabolic resistance to DDT in the malaria vector Anopheles funestus. Genome-wide transcription analysis revealed that GSTe2 was the most over-expressed detoxification gene in DDT and permethrin-resistant mosquitoes from Benin. Transgenic expression of GSTe2 in Drosophila melanogaster demonstrated that over-transcription of this gene alone confers DDT resistance and cross-resistance to py

SUBMITTER: Riveron JM 

PROVIDER: S-EPMC4054843 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature