A high density physical map of chromosome 1BL supports evolutionary studies, map-based cloning and sequencing in wheat.

ABSTRACT:

Background

As for other major crops, achieving a complete wheat genome sequence is essential for the application of genomics to breeding new and improved varieties. To overcome the complexities of the large, highly repetitive and hexaploid wheat genome, the International Wheat Genome Sequencing Consortium established a chromosome-based strategy that was validated by the construction of the physical map of chromosome 3B. Here, we present improved strategies for the construction of highly integrated and ordered wheat physical maps, using chromosome 1BL as a template, and illustrate their potential for evolutionary studies and map-based cloning.

Results

Using a combination of novel high throughput marker assays and an assembly program, we developed a high quality physical map r

SUBMITTER: Philippe R 

PROVIDER: S-EPMC4054855 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature