Discovery of novel mutations for clarithromycin resistance in Helicobacter pylori by using next-generation sequencing.

ABSTRACT:

Objectives

Resistance to clarithromycin is the most important factor causing failure of Helicobacter pylori eradication. Although clarithromycin resistance is mainly associated with three point mutations in the 23S rRNA genes, it is unclear whether other mutations are associated with this resistance.

Methods

Two types of clarithromycin-resistant strains (low- and high-resistance strains) were obtained from clarithromycin-susceptible H. pylori following exposure to low clarithromycin concentrations. The genome sequences were determined with a next-generation sequencer. Natural transformation was used to introduce the candidate mutations into strain 26695. Etest and an agar dilution method were used to determine the MICs.

Results

High-resistance strains contained the mu

SUBMITTER: Binh TT 

PROVIDER: S-EPMC4054984 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature