The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional.

ABSTRACT:

Introduction

We previously demonstrated that the lifespan of primary human keratinocytes could be extended indefinitely by culture in the presence of the Rho kinase (ROCK) inhibitor Y-27632. This technique has proven to be very useful in diverse areas of basic and clinical research.

Methods

In this follow-up study we determine whether the continual presence of Y-27632 is required for sustained proliferation. We also test whether different ROCK inhibitors can be used for this technique and whether it can also promote indefinite proliferation of animal keratinocytes. We measure keratinocyte gene expression, proliferation, behaviour and lifespan in the presence and absence of Y-27632.

Results

We demonstrate that the extension of lifespan observed by culture of keratinocy

SUBMITTER: Chapman S 

PROVIDER: S-EPMC4055106 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature