Human placenta-derived neurospheres are susceptible to transformation after extensive in vitro expansion.

ABSTRACT:

Introduction

The cancer stem cell model links neoplastic cells with normal stem cell biology, but little is known on how normal stem cells are transformed into cancer stem cells.

Methods

To investigate the processes underlying the transformation of normal stem cells we developed in vitro a cancer stem cell model from human amniotic and chorionic placenta membranes. In this model we studied the expression of specific stem cell molecules by flow cytometry, and genes, by real time RT-PCR. Microscopy immunfluorescence was employed to investigate the proliferative and differentiation patterns. Fluorescence microscopy and FACS were employed to investigate the proliferative and differentiation patterns. To evaluate the tumorigenic potential of our model we injected the cells into N

SUBMITTER: Amendola D 

PROVIDER: S-EPMC4055136 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature