Gene markers of cellular aging in human multipotent stromal cells in culture.

ABSTRACT:

Introduction

Human multipotent stromal cells (MSCs) isolated from bone marrow or other tissue sources have great potential to treat a wide range of injuries and disorders in the field of regenerative medicine and tissue engineering. In particular, MSCs have inherent characteristics to suppress the immune system and are being studied in clinical studies to prevent graft-versus-host disease. MSCs can be expanded in vitro and have potential for differentiation into multiple cell lineages. However, the impact of cell passaging on gene expression and function of the cells has not been determined.

Methods

Commercially available human MSCs derived from bone marrow from six different donors, grown under identical culture conditions and harvested at cell passages 3, 5, and 7, were an

SUBMITTER: Bellayr IH 

PROVIDER: S-EPMC4055144 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature