PPARβ activation restores the high glucose-induced impairment of insulin signalling in endothelial cells.

ABSTRACT:

Background and purpose

PPARβ enhances insulin sensitivity in adipocytes and skeletal muscle cells, but its effects on insulin signalling in endothelial cells are not known. We analysed the effects of the PPARβ/δ (PPARβ) agonists, GW0742 and L165041, on impaired insulin signalling induced by high glucose in HUVECs and aortic and mesenteric arteries from diabetic rats.

Experimental approach

Insulin-stimulated NO production, Akt-Ser(473) and eNOS-Ser(1177) phosphorylation, and reactive oxygen species (ROS) production were studied in HUVECs incubated in low- or high-glucose medium. Insulin-stimulated relaxations and protein phosphorylation in vessels from streptozotocin (STZ)-induced diabetic rats were also analysed.

Key results

HUVECs incubated in high-glucose medium sho

SUBMITTER: Quintela AM 

PROVIDER: S-EPMC4055208 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature