Reliable assessment and quantification of the fluorescence-labeled antisense oligonucleotides in vivo.

ABSTRACT: The availability of fluorescent dyes and the advances in the optical systems for in vivo imaging have stimulated an increasing interest in developing new methodologies to study and quantify the biodistribution of labeled agents. However, despite these great achievements, we are facing significant challenges in determining if the observed fluorescence does correspond to the quantity of the dye in the tissues. In fact, although the far-red and near-infrared lights can propagate through several centimetres of tissue, they diffuse within a few millimetres as consequence of the elastic scattering of photons. In addition, when dye-labeled oligonucleotides form stable complex with cationic carriers, a large change in the fluorescence intensity of the dye is observed. Therefore, the measured fluor

SUBMITTER: Munisso MC 

PROVIDER: S-EPMC4055493 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature