A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei.

ABSTRACT: In trypanosomatids, gene expression is regulated mainly by post-transcriptional mechanisms, which affect mRNA processing, translation and degradation. Currently, our understanding of factors that regulate either mRNA stability or translation is rather limited. We know that often, the regulators are proteins that bind to the 3'-untranslated region; they presumably interact with ribonucleases and translation factors. However, very few such proteins have been characterized in any detail. Here we describe a genome-wide screen to find proteins implicated in post-transcriptional regulation in Trypanosoma brucei. We made a library of random genomic fragments in a plasmid that was designed for expression of proteins fused to an RNA-binding domain, the lambda-N peptide. This was transfected into ce

SUBMITTER: Erben ED 

PROVIDER: S-EPMC4055773 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature