A method to identify and validate mitochondrial modulators using mammalian cells and the worm C. elegans.

ABSTRACT: Mitochondria are semi-autonomous organelles regulated by a complex network of proteins that are vital for many cellular functions. Because mitochondrial modulators can impact many aspects of cellular homeostasis, their identification and validation has proven challenging. It requires the measurement of multiple parameters in parallel to understand the exact nature of the changes induced by such compounds. We developed a platform of assays scoring for mitochondrial function in two complementary models systems, mammalian cells and C. elegans. We first optimized cell culture conditions and established the mitochondrial signature of 1,200 FDA-approved drugs in liver cells. Using cell-based and C. elegans assays, we further defined the metabolic effects of two pharmacological classes that emerg

SUBMITTER: Andreux PA 

PROVIDER: S-EPMC4055904 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature