Genomic occupancy of Runx2 with global expression profiling identifies a novel dimension to control of osteoblastogenesis.

ABSTRACT:

Background

Osteogenesis is a highly regulated developmental process and continues during the turnover and repair of mature bone. Runx2, the master regulator of osteoblastogenesis, directs a transcriptional program essential for bone formation through genetic and epigenetic mechanisms. While individual Runx2 gene targets have been identified, further insights into the broad spectrum of Runx2 functions required for osteogenesis are needed.

Results

By performing genome-wide characterization of Runx2 binding at the three major stages of osteoblast differentiation--proliferation, matrix deposition and mineralization--we identify Runx2-dependent regulatory networks driving bone formation. Using chromatin immunoprecipitation followed by high-throughput sequencing over the course of

SUBMITTER: Wu H 

PROVIDER: S-EPMC4056528 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature