Repression of chimeric transcripts emanating from endogenous retrotransposons by a sequence-specific transcription factor.

ABSTRACT:

Background

Retroviral elements are pervasively transcribed and dynamically regulated during development. While multiple histone- and DNA-modifying enzymes have broadly been associated with their global silencing, little is known about how the many diverse retroviral families are each selectively recognized.

Results

Here we show that the zinc finger protein Krüppel-like Factor 3 (KLF3) specifically silences transcription from the ORR1A0 long terminal repeat in murine fetal and adult erythroid cells. In the absence of KLF3, we detect widespread transcription from ORR1A0 elements driven by the master erythroid regulator KLF1. In several instances these aberrant transcripts are spliced to downstream genic exons. One such chimeric transcript produces a novel, dominant negative is

SUBMITTER: Mak KS 

PROVIDER: S-EPMC4056533 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature