Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.

ABSTRACT:

Objective

Differential gene expression in CD177+ and CD177- neutrophils was investigated, in order to detect possible differences in neutrophil function which could be related to the pathogenesis of ANCA-associated Vasculitides (AAV).

Methods

Neutrophils were isolated from healthy controls (HC) with high, negative or bimodal CD177 expression, and sorted into CD177+ and CD177- subpopulations. Total RNA was screened for expression of 24,000 probes with Illumina Ref-8 Beadchips. Genes showing differential expression between CD177+ and CD177- subsets in microarray analysis were re-assessed using quantitative-PCR. CD177 expression on neutrophil precursors in bone marrow was analyzed using quantitative PCR and flowcytometry.

Results

The proportion of CD177+ cells increased

SUBMITTER: Hu N 

PROVIDER: S-EPMC4057222 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature