Quantification of functionalised gold nanoparticle-targeted knockdown of gene expression in HeLa cells.

ABSTRACT:

Introduction

Gene therapy continues to grow as an important area of research, primarily because of its potential in the treatment of disease. One significant area where there is a need for better understanding is in improving the efficiency of oligonucleotide delivery to the cell and indeed, following delivery, the characterization of the effects on the cell.

Methods

In this report, we compare different transfection reagents as delivery vehicles for gold nanoparticles functionalized with DNA oligonucleotides, and quantify their relative transfection efficiencies. The inhibitory properties of small interfering RNA (siRNA), single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA) sequences targeted to human metallothionein hMT-IIa are also quantified in HeLa cells. Techniqu

SUBMITTER: Jiwaji M 

PROVIDER: S-EPMC4057226 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature