Differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition.

ABSTRACT:

Background

Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian × Landrace backcross with extreme phenotypes according to FA profile.

Results

We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic region

SUBMITTER: Puig-Oliveras A 

PROVIDER: S-EPMC4057286 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature