Frequent co-expression of miRNA-5p and -3p species and cross-targeting in induced pluripotent stem cells.

ABSTRACT:

Background

A miRNA precursor generally gives rise to one major miRNA species derived from the 5' arm, and are called miRNA-5p. However, more recent studies have shown co-expression of miRNA-5p and -3p, albeit in different concentrations, in cancer cells targeting different sets of transcripts. Co-expression and regulation of the -5p and -3p miRNA species in stem cells, particularly in the reprogramming process, have not been studied.

Methods

In this work, we investigated co-expression and regulation of miRNA-5p and -3p species in human induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and embryonic stem cells (ESC) using a nanoliter-scale real-time PCR microarray platform that included 1,036 miRNAs.

Results

In comparing iPSC and ESC, only 32 miRNAs

SUBMITTER: Huang CJ 

PROVIDER: S-EPMC4057479 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature