Efficient delivery of plasmid DNA using cholesterol-based cationic lipids containing polyamines and ether linkages.

ABSTRACT: Cationic liposomes are broadly used as non-viral vectors to deliver genetic materials that can be used to treat various diseases including cancer. To circumvent problems associated with cationic liposome-mediated delivery systems such as low transfection efficiency and serum-induced inhibition, cholesterol-based cationic lipids have been synthesized that resist the effects of serum. The introduction of an ether-type linkage and extension of the aminopropyl head group on the cholesterol backbone increased the transfection efficiency and DNA binding affinity compared to a carbamoyl-type linkage and a mono aminopropyl head group, respectively. Under optimal conditions, each liposome formulation showed higher transfection efficiency in AGS and Huh-7 cells than commercially available cationic l

SUBMITTER: Kim BK 

PROVIDER: S-EPMC4057673 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature