A proposed mechanism for IS607-family serine transposases.

ABSTRACT:

Background

The transposases encoded by the IS607 family of mobile elements are unusual serine recombinases with an inverted domain order and minimal specificity for target DNA.

Results

Structural genomics groups have determined three crystal structures of the catalytic domains of IS607 family transposases. The dimers formed by these catalytic domains are very different from those seen for other serine recombinases and include interactions that usually only occur upon formation of a synaptic tetramer.

Conclusions

Based on these structures, we propose a model for how IS607-family transposases could form a synaptic tetramer. The model suggests that, unlike other serine recombinases, these enzymes carry out sequence-specific DNA binding and catalysis in trans: the DNA bin

SUBMITTER: Boocock MR 

PROVIDER: S-EPMC4058570 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature