FRNK inhibition of focal adhesion kinase-dependent signaling and migration in vascular smooth muscle cells.

ABSTRACT:

Objective

To examine whether interference with FRNK targeting to focal adhesions (FAs) affects its inhibitory activity and tyrosine phosphorylation.

Methods and results

Focal adhesion kinase and its autonomously expressed C-terminal inhibitor, focal adhesion kinase-related nonkinase (FRNK), regulate vascular smooth muscle cell (VSMC) signaling and migration. FRNK-paxillin binding was reduced by a point mutation in its FA targeting domain (L341S-FRNK). Green fluorescent protein-tagged wild type and L341S-FRNK were then adenovirally expressed in VSMCs. L341S-FRNK targeted to VSMC FAs, despite previous studies in other cell types. L341S-FRNK affected FA binding kinetics (assessed by total internal reflection fluorescnece [TIRF] microscopy and fluorescence recovery after photobl

SUBMITTER: Koshman YE 

PROVIDER: S-EPMC4058887 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature