Label-free determination of hemodynamic parameters in the microcirculaton with third harmonic generation microscopy.

ABSTRACT: Determination of blood flow velocity and related hemodynamic parameters is an important aspect of physiological studies which in many settings requires fluorescent labeling. Here we show that Third Harmonic Generation (THG) microscopy is a suitable tool for label-free intravital investigations of the microcirculation in widely-used physiological model systems. THG microscopy is a non-fluorescent multi-photon scanning technique combining the advantages of label-free imaging with restriction of signal generation to a focal spot. Blood flow was visualized and its velocity was measured in adult mouse cremaster muscle vessels, non-invasively in mouse ear vessels and in Xenopus tadpoles. In arterioles, THG line scanning allowed determination of the flow pulse velocity curve and hence the heart r

SUBMITTER: Dietzel S 

PROVIDER: S-EPMC4059650 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature