Ontology highlight
ABSTRACT:
SUBMITTER: Tsai A
PROVIDER: S-EPMC4059775 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Cell reports 20140515 5
SecM is an E. coli secretion monitor capable of stalling translation on the prokaryotic ribosome without cofactors. Biochemical and structural studies have demonstrated that the SecM nascent chain interacts with the 50S subunit exit tunnel to inhibit peptide bond formation. However, the timescales and pathways of stalling on an mRNA remain undefined. To provide a dynamic mechanism for stalling, we directly tracked the dynamics of elongation on ribosomes translating the SecM stall sequence (FSTPV ...[more]