Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies.

ABSTRACT: Single particle cryo-electron microscopy (cryo-EM) is an emerging powerful tool for structural studies of macromolecular assemblies (i.e., protein complexes and viruses). Although single particle cryo-EM requires less concentrated and smaller amounts of samples than X-ray crystallography, it remains challenging to study specimens that are low-abundance, low-yield, or short-lived. The recent development of affinity grid techniques can potentially further extend single particle cryo-EM to these challenging samples by combining sample purification and cryo-EM grid preparation into a single step. Here we report a new design of affinity cryo-EM approach, cryo-SPIEM, that applies a traditional pathogen diagnosis tool Solid Phase Immune Electron Microscopy (SPIEM) to the single particle cryo-EM m

SUBMITTER: Yu G 

PROVIDER: S-EPMC4060259 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature