Multi-wavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors.

ABSTRACT: Photoacoustic imaging is an emerging hybrid imaging technology capable of breaking through resolution limits of pure optical imaging technologies imposed by optical-scattering to provide fine-resolution optical contrast information in deep tissues. We demonstrate the ability of multi-wavelength photoacoustic imaging to estimate relative gene expression distributions using an inducible expression system and co-register images with hemoglobin oxygen saturation estimates and micro-ultrasound data. Tyrosinase, the rate-limiting enzyme in melanin production, is used as a reporter gene owing to its strong optical absorption and enzymatic amplification mechanism. Tetracycline-inducible melanin expression is turned on via doxycycline treatment in vivo. Serial multi-wavelength imaging reveals very

SUBMITTER: Paproski RJ 

PROVIDER: S-EPMC4060505 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature