Hypochlorite-modified high-density lipoprotein acts as a sink for myeloperoxidase in vitro.

ABSTRACT:

Aims

Myeloperoxidase (MPO), a cardiovascular risk factor in humans, is an in vivo catalyst for lipoprotein modification via intermediate formation of reactive chlorinating species. Among the different lipoprotein classes, anti-atherogenic high-density lipoprotein (HDL) represents a major target for modification by hypochlorous acid (HOCl), generated from H2O2 by MPO in the presence of physiological chloride concentrations. As MPO was identified as an HDL-associated protein that could facilitate selective oxidative modification of its physiological carrier, the aim of the present study was to investigate whether and to what extent modification of HDL by HOCl affects the binding affinity of MPO in vitro.

Methods and results

We show that binding affinity of 125I-labelled MPO to

SUBMITTER: Marsche G 

PROVIDER: S-EPMC4060512 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature