Targeted next-generation sequencing at copy-number breakpoints for personalized analysis of rearranged ends in solid tumors.

ABSTRACT:

Background

The concept of the utilization of rearranged ends for development of personalized biomarkers has attracted much attention owing to its clinical applicability. Although targeted next-generation sequencing (NGS) for recurrent rearrangements has been successful in hematologic malignancies, its application to solid tumors is problematic due to the paucity of recurrent translocations. However, copy-number breakpoints (CNBs), which are abundant in solid tumors, can be utilized for identification of rearranged ends.

Method

As a proof of concept, we performed targeted next-generation sequencing at copy-number breakpoints (TNGS-CNB) in nine colon cancer cases including seven primary cancers and two cell lines, COLO205 and SW620. For deduction of CNBs, we developed a novel

SUBMITTER: Kim HK 

PROVIDER: S-EPMC4061055 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature