Polymorphic Protein Crystal Growth: Influence of Hydration and Ions in Glucose Isomerase.

ABSTRACT: Crystal polymorphs of glucose isomerase were examined to characterize the properties and to quantify the energetics of protein crystal growth. Transitions of polymorph stability were measured in poly(ethylene glycol)/NaCl solutions, and one transition point was singled out for more detailed quantitative analysis. Single crystal x-ray diffraction was used to confirm space groups and identify complementary crystal structures. Crystal polymorph stability was found to depend on the NaCl concentration, with stability transitions requiring > 1 M NaCl combined with a low concentration of PEG. Both salting-in and salting-out behavior was observed and was found to differ for the two polymorphs. For NaCl concentrations above the observed polymorph transition, the increase in solubility of the less s

SUBMITTER: Gillespie CM 

PROVIDER: S-EPMC4061714 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature