Peripheral nerve morphogenesis induced by scaffold micropatterning.

ABSTRACT: Several bioengineering approaches have been proposed for peripheral nervous system repair, with limited results and still open questions about the underlying molecular mechanisms. We assessed the biological processes that occur after the implantation of collagen scaffold with a peculiar porous micro-structure of the wall in a rat sciatic nerve transection model compared to commercial collagen conduits and nerve crush injury using functional, histological and genome wide analyses. We demonstrated that within 60 days, our conduit had been completely substituted by a normal nerve. Gene expression analysis documented a precise sequential regulation of known genes involved in angiogenesis, Schwann cells/axons interactions and myelination, together with a selective modulation of key biological p

SUBMITTER: Cerri F 

PROVIDER: S-EPMC4061729 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature