Early aberrant DNA methylation events in a mouse model of acute myeloid leukemia.

ABSTRACT:

Background

Aberrant DNA methylation is frequently found in human malignancies including acute myeloid leukemia (AML). While most studies focus on later disease stages, the onset of aberrant DNA methylation events and their dynamics during leukemic progression are largely unknown.

Methods

We screened genome-wide for aberrant CpG island methylation in three disease stages of a murine AML model that is driven by hypomorphic expression of the hematopoietic transcription factor PU.1. DNA methylation levels of selected genes were correlated with methylation levels of CD34+ cells and lineage negative, CD127-, c-Kit+, Sca-1+ cells; common myeloid progenitors; granulocyte-macrophage progenitors; and megakaryocyte-erythroid progenitors.

Results

We identified 1,184 hypermethylat

SUBMITTER: Sonnet M 

PROVIDER: S-EPMC4062060 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature