Deciphering the cis-regulatory elements for XYR1 and CRE1 regulators in Trichoderma reesei.

ABSTRACT: In this work, we report the in silico identification of the cis-regulatory elements for XYR1 and CRE1 proteins in the filamentous fungus Trichoderma reesei, two regulators that play a central role in the expression of cellulase genes. Using four datasets of condition-dependent genes from RNA-seq and RT-qPCR experiments, we performed unsupervised motif discovery and found two short motifs resembling the proposed binding consensus for XYR1 and CRE1. Using these motifs, we analysed the presence and arrangement of putative cis-regulatory elements recognized by both regulators and found that shortly spaced sites were more associated with XYR1- and CRE1-dependent promoters than single, high-score sites. Furthermore, the approach used here allowed the identification of the previously reported XYR

SUBMITTER: Silva-Rocha R 

PROVIDER: S-EPMC4062390 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature