A genetically encoded aldehyde for rapid protein labelling.

ABSTRACT: Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

SUBMITTER: Tuley A 

PROVIDER: S-EPMC4062584 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature